2006 Philadelphia Annual Meeting (22–25 October 2006)

Session No. 7
Sunday, 22 October 2006: 8:00 AM-12:00 PM

T29. The Use of Molecular Techniques to Assess Microbial Community Structure and Function in Aquifer Systems

GSA Geobiology & Geomicrobiology Division; GSA Hydrogeology Division

 

Johanna V. Weiss, Isabelle M. Cozzarelli and Brian Mailloux, Advocates
Paper #
Start Time
 
8:00 AM
Introductory Remarks
1
8:05 AM
AQUIFER VS. COAL-DERIVED METHANOGENS IN PALEOCENE FORT UNION FORMATION COALS – MOLECULAR TECHNIQUE-BASED INFORMATION AND ITS ECOLOGICAL IMPLICATIONS
KLEIN, D.A.1, FLORES, R.M.2, VENOT, C.3, PEREYRA, L.P.3 and PRUDEN, A.3, (1)Microbiology, Immunology and Pathology, Colorado State Univ, 1682 Campus Delivery, Fort Collins, CO 805231682, (2)U.S. GEOLOGICAL SURVEY, Denver Federal Center, MS939, P.O. Box 25046, Denver, CO 80225, (3)Civil and Environmental Engineering, Colorado State Univ, Fort Collins, CO 80523, dakspk@lamar.colostate.edu, dakspk@lamar.colostate.edu
2
8:20 AM
16S RRNA GENE LIBRARIES TO CHARACTERIZE THE MICROBIAL COMMUNITY STRUCTURE AT PETROLEUM-CONTAMINATED AQUIFERS EXHIBITING ANOMALOUS HIGH ELECTRICAL CONDUCTIVITIES
ROSSBACH, Silvia1, ALLEN, Jonathan P.1, ATEKWANA, Estella A.2 and ATEKWANA, Eliot A.2, (1)Biological Sciences, Western Michigan University, Kalamazoo, MI 49008-5410, (2)School of Geology, Oklahoma State University, Noble Research Center, Stillwater, OK 74078, Silvia.Rossbach@wmich.edu, Silvia.Rossbach@wmich.edu
3
8:35 AM
GROUNDWATER FILTRATES AS SAMPLES OF THE SUBSURFACE MICROBIAL COMMUNITY
FLYNN, Theodore M.1, SANFORD, Robert A.2, WALLACE, Paige L.2 and BETHKE, Craig M.2, (1)Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, (2)Department of Geology, University of Illinois, 1301 W. Green St, Urbana, IL 61801, tmflynn2@uiuc.edu, tmflynn2@uiuc.edu
4
8:50 AM
DEVELOPMENT AND IMPLEMENTATION OF BACTERIAL ISOLATES EXPRESSING BLUE AND GREEN FLUORESCING PROTEINS (BFP AND GFP) AS ALTERNATIVES TO CONVENTIONAL DNA- AND PROTEIN-SPECIFIC FLUORESCENCE STAINING FOR STUDYING BACTERIAL TRANSPORT BEHAVIOR IN GRANULAR MEDIA
METGE, David W.1, WANG, Meng2, FORD, Roseanne M.2, TOEPFER, J. Amanda2 and HARVEY, Ronald W.1, (1)US Geological Survey, 3215 Marine, Marine Street Science Ctr, Boulder, CO 80303, (2)Chemical Engineering, University of Virginia, 102 Engineers' Way, P.O. Box 400741, Charlottesville, VA 22904-4741, dwmetge@usgs.gov, dwmetge@usgs.gov
5
9:05 AM
USING MICROBIAL COMMUNITY PROFILES FOR DELINEATION AND LONG-TERM MONITORING OF A LANDFILL LEACHATE-CONTAMINATED AQUIFER
MOUSER, Paula J.1, RIZZO, Donna M.1, O'GRADY, Patrick2, DRUSCHEL, Gregory3, HAYDEN, Nancy1 and STEVENS, Lori4, (1)Civil and Environmental Engineering, University of Vermont, 213 Votey Building, Burlington, VT 05405, (2)Department of Environmental Science, Policy, and Management, University of California, Berkeley, 125A Hilgard, Berkeley, CA 94720, (3)Department of Geology, University of Vermont, 321 Delehanty Hall, Burlington, VT 05405, (4)Department of Biology, University of Vermont, 318 Marsh Life Sciences Building, Burlington, VT 05405, Paula.Mouser@uvm.edu, Paula.Mouser@uvm.edu
6
9:20 AM
THE USE OF QUANTITATIVE PCR TO EXAMINE CONTROLS ON BIOGEOCHEMICAL PROCESSES AT AN AQUIFER CONTAMINATED WITH LANDFILL LEACHATE (NORMAN,OK)
WEISS, Johanna V., VOYTEK, Mary A., KIRSHTEIN, Julie D. and COZZARELLI, Isabelle M., U.S. Geological Survey, 431 National Center, Reston, VA 20192, jweiss@usgs.gov, jweiss@usgs.gov
 
9:35 AM
Break
7
9:50 AM
MONITORING THE METABOLIC STATE OF GEOBACTERACEAE DURING IN SITU U(VI) BIOREMEDIATION
HOLMES, Dawn E., O'NEIL, Regina A. and LOVLEY, Derek R., Microbiology, University of Massachusetts, Amherst, Morrill IV North Science Center, Amherst, MA 01003, dholmes@microbio.umass.edu, dholmes@microbio.umass.edu
8
10:10 AM
METHODS FOR DETECTION OF ARSENIC METABOLIZING BACTERIA
STOLZ, John F.1, FISHER, Edward1, POLSHNYA, Ganna2, BEREKAA, Mahmoud3, GARCIA, Antonio4, THANGAVELUM, Miru1 and BASU, Partha2, (1)Biological Sciences, Duquesne University, 600 Forbes Avenue, Pittsburgh, PA 15282, (2)Department of Chemistry and Biochemistry, Duquesne University, 600 Forbes Avenue, Pittsburgh, PA 15282, (3)Environmental Sciences, University of Alexandria, Alexandria, Egypt, (4)Departmento de Biologica Molecular, Universidad Autonoma de Madrid, Madrid, Spain, stolz@duq.edu, stolz@duq.edu
9
10:30 AM
LINKING BACTERIAL ACTIVITY AND DIVERSITY IN THE SHALLOW AQUIFERS OF BANGLADESH
MAILLOUX, Brian J.1, VAN GEEN, A.2, ZHENG, Yan3, STUTE, M.2, DATTA, Saugata4, HORNEMAN, A.5, MISLOWACK, B.J.6, DONG, Hailiang7, FREYER, Greg8 and ASSAF-ANID, Nada9, (1)Environmental Science, Barnard College, 76 Claremont Ave, 404 Altschul Hall, New York, NY 10027, (2)Lamont-Doherty Earth Observatory of Columbia Univ, 61 Rte. 9W, Palisades, NY 10964, (3)Earth and Environmental Sciences, Queens College and Graduate Center, CUNY, 65-30 Kissena Blvd, Flushing, NY 11367, (4)Biological & Environmental Sciences, Georgia College and State University, 204 Herty Hall, Milledgeville, GA 31061-0490, (5)Department of Earth and Environmental Engineering, Columbia University, 918 Seeley Mudd Building, 500 West 120th St, New York, NY 10027, (6)Geosciences, Princeton Univ, Guyot Hall, Washington Road, Princeton, NJ 08544-1003, (7)Department of Geology, Miami University, 114 Shideler Hall, Oxford, OH 45056, (8)Environmental Health Science, Columbia University, New York, NY 10027, (9)Manhattan College, Riverdale, NY 10471, bjm2103@columbia.edu, bjm2103@columbia.edu
10
10:45 AM
THE SEARCH FOR LIFE IN THE DEEP SUBSURFACE: MEMBRANE LIPID BIOSIGNATURES
PFIFFNER, Susan M., Indiana Princeton Tennessee Astrobiology Initiative (IPTAI), Unviersity of Tennessee, Center for Biomarker Analysis, 10515 Research Dr., Ste. 300, Knoxville, TN 37932, WHITE, David C., University of Tennessee, Center for Biomarker Analysis, Knoxville, TN 37932, PHELPS, Tommy J., Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, PRATT, Lisa, IPTAI, Dept. of Geological Sciences, Indiana University, Bloomington, IN 47405, ONSTOTT, T.C., IPTAI, Dept. of Geosciences, Princeton University, Princeton, NJ 08544 and VAN HEERDEN, Esta, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa, pfiffner@utk.edu, pfiffner@utk.edu
11
11:05 AM
LIPID CHARACTERIZATION OF MICROBIAL COMMUNITIES IN GROUNDWATER ADJACENT TO A FRESHWATER LAKE IN NORTH-CENTRAL IOWA
FANG, Jiasong1, SIMPKINS, William W.2, DAS GUPTA, Shamik2 and WERNE, Josef3, (1)Geological and Atmospheric Sciences, Iowa State Univ, 360 Science I, Ames, IA 50011, (2)Department of Geological and Atmospheric Sciences, Iowa State University, 253 Science I, Iowa State University, Ames, IA 50011, (3)Department of Chemistry and Biochemistry, University of Minnesota Duluth, Duluth, MN 55812, jsfang@iastate.edu, jsfang@iastate.edu
12
11:20 AM
VOLATILE ORGANIC CARBON METABOLITES AS INDICATORS OF SOIL MICROBIAL COMMUNITY SHIFT IN MICROCOSM STUDIES
MCNEAL, Karen S., Geology & Geophysics, Texas A&M University, 8502 Easton Commons Dr, Apt #104, Houston, TX 77095 and HERBERT, Bruce E., Geology & Geophysics, Texas A&M Univ, College Station, TX 77843-3115, ksell@neo.tamu.edu, ksell@neo.tamu.edu
13
11:35 AM
BIOGEOCHEMISTRY AND MOLECULAR MICROBIAL ECOLOGY OF GREAT SULPHUR SPRING, WESTERN LAKE ERIE, MICHIGAN
HAACK, Sheridan Kidd, US Geological Survey, Michigan Water Science Center, 6520 Mercantile Way, Suite 5, Lansing, MI 48911, skhaack@usgs.gov, skhaack@usgs.gov
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