RAPID IN-SITU RESIN IMPREGNATION OF UNCONSOLIDATED AEOLIAN SAND DUNES
The sampling site is located in a remote area without electricity, therefore it was not possible to impregnate the samples under vacuum, as would typically be done in a laboratory setting. I selected a surfboard resin that could be thinned to an extremely low viscosity, with a short-but-modifiable gel-time. To prevent the resins rapid dispersal in the unconsolidated sand and gravel of the anthills I carefully inserted a tin can with one end removed into the anthill. I then used a syringe to inject the resin through a small hole drilled in the closed end of the can, and left the sample to harden overnight. The samples were not completely cured, but they had hardened enough to be transported without any detectable damage to the anthills structure. A secondary impregnation conducted using a vacuum oven and the same resin mixture was sufficient to allow the samples to be sliced open for thin sectioning.
This technique has broad applications for study of bio-sedimentary structures, as the short gel time prevents the breakdown of organic matter which can occur with other resin impregnation techniques. My focus is on ants, but the macro-structure of a microbiological community could also be studied using this method.