|Southeastern Section - 54th Annual Meeting (March 17–18, 2005)|
|Paper No. 1-5|
|Presentation Time: 9:30 AM-9:50 AM|
POSSIBLE DNA PRESERVATION FROM PLANT FOSSILS IN THE CLAIBORNE FORMATION (MIDDLE EOCENE) OF WEST TENNESSEE
GIBSON, Michael A., Dept. of Geology, Geography, and Physics, Univ of Tennessee at Martin, 215 Joseph E. Johnson EPS Bldg, Martin, TN 38238, firstname.lastname@example.org, CARLSON, John E., Schatz Center for Tree Molecular Genetics, Pennsylvania State Univ, 405C Life Sciences Bldg, University Park, PA 16802, and SCHLARBAUM, Scott E., Dept. of Forestry, Wildlife, & Fisheries, Univ of Tennessee Knoxville, Institute of Agriculture, Knoxville, TN 37996|
The Claiborne Formation in Tennessee is composed of massive bedded, dark gray to black, organic-rich clay lenses and surrounding sands interpreted as oxbow lakes and fluvial channels of a floodplain-river complex in West Tennessee during the Middle Eocene. The Claiborne is well known for its pristine preservation and diversity of fossil leaves, flowers containing pollen, and associated insects. Individual leaves can be peeled and studied using techniques employed on modern leaves (e.g., stomatal counting, margin analysis, etc.) and pollen is extracted from individual flowers. The preservation of intact and amplifiable DNA from the Eocene Claiborne indicates fossil preservation as a type of Konservat Lagerstätten. As part of a project focusing on the reintroduction/revitalization of living Chestnut in Tennessee, fossilized indigenous Chestnut was sought for comparison. During this search we determined that the preservation state of Claiborne fossil leaves might be favorable for DNA recovery. We have had promising results with standard protocols for DNA extraction which yielded intact DNA. Additional DNA extraction protocols are being tested, to provide DNA that is also suitable for PCR amplification and DNA sequencing. Successful DNA extraction from Claiborne fossil leaves can provide benchmarks for phylogenetic and evolutionary studies between Eocene and living plants, thus allowing a linkage of systematics to a level not generally realized in Eocene deposits. Specifically, DNA analysis should result in the correct taxonomic placement of extinct species within the Claiborne.
Southeastern Section - 54th Annual Meeting (March 17–18, 2005)
General Information for this Meeting
|Session No. 1|
Exceptional Biotas and Fossil Preservation in the Southeast
Bayview Hotel at the Grand Casino Resort: 1
8:00 AM-12:40 PM, Thursday, March 17, 2005
Geological Society of America Abstracts with Programs, Vol. 37, No. 2, p. 4
© Copyright 2005 The Geological Society of America (GSA), all rights reserved. Permission is hereby granted to the author(s) of this abstract to reproduce and distribute it freely, for noncommercial purposes. Permission is hereby granted to any individual scientist to download a single copy of this electronic file and reproduce up to 20 paper copies for noncommercial purposes advancing science and education, including classroom use, providing all reproductions include the complete content shown here, including the author information. All other forms of reproduction and/or transmittal are prohibited without written permission from GSA Copyright Permissions.