DELINEATING SPECIES OF RAFINESQUINA IN THE TYPE CINCINNATIAN (ORDOVICIAN): A MORPHOMETRIC APPROACH
The great abundance of available fossils makes the genus Rafinesquina an ideal subject on which to test the efficacy of geometric morphometrics as a method of species delineation. Two-dimensional geometric morphometrics was used to determine the number of Rafinesquina species present in the Type Cincinnatian. Dorsal and ventral valve exteriors of 13 previously named species from the Cincinnatian Series were digitized and analyzed using a combination of landmark, semi-landmark and outline based analyses.
Landmark and outline data were captured using ImageJ. The landmark data were analyzed with Geomorph, and the outline data were analyzed with SHAPE v1.3. General Procrustes Analysis (GPA) was performed on both data sets to remove size as a variable. Principal Component Analysis (PCA) was used to rotate the data onto major axes and remove correlation between variables and identify the Principal Components (shape variables) for further analysis. The principal components with the highest loading (PC1 and PC2) were visualized using regression methods. Statistical analyses were then used to identify discrete species morphologies within morphospace.
Based on results of this study, two-dimensional geometric morphometrics is a successful tool for identifying distinct species of Rafinesquina in the study region. These analyses can provide the basis for differentiating population-level variation from species-level distinctions required for future systematic revision of the genus.