GSA Annual Meeting in Indianapolis, Indiana, USA - 2018

Paper No. 62-12
Presentation Time: 4:15 PM

SWIM-IRMS: METHODS IN EXPLORING ISOTOPE COMPOSITION IN MICROFOSSILS, AND ITS APPLICATION IN PALEOECOLOGY (Invited Presentation)


URBAN, Michael A.1, NELSON, David M.2, CLEGG, Benjamin3, ROMERO, Ingrid1, ZHAO, Yongtao4, PUNYASENA, Surangi W.1 and HU, Feng Sheng1, (1)Department of Plant Biology, University of Illinois, 505 S. Goodwin Ave., Urbana, IL 61801, (2)University of Maryland, Center for Environmental Science, 301 Braddock Road, Frostburg, MD 21532, (3)School of Integrative Biology, University of Illinois, 505 S. Goodwin Ave., Urbana, IL 61801, (4)College of Earth and Environmental Sciences, MOE Key Laboratory of Western China's Environmental Systems, Lanzhou University, Lanzhou, 730000, China

The limitation of available substrate for analyses is a major hurdle in the use of stable carbon isotopes in micropaleontology. Traditional techniques (e.g., EA-IRMS) can be time consuming and require substantial fossil material that is often unavailable. To combat these issues, we developed techniques using a spooling wire microcombustion device (SWiM) were interfaced with IRMS to explore paleoecological questions that were previously difficult to answer. One example is the evolution and expansion of C4 grasses. Using SWiM-IRMS, single pollen isotope AnaLysis (SPIRAL) was developed to measure nanomolar amounts of carbon in single pollen grains and estimate the abundance and probability of occurrence of C4 grasses. We were able to show with SPIRAL the lack of C4 grasses in the Eocene of southeastern North America, the earliest presence of C4 grasses in the Oligocene of western Europe, and that C4 grasses were relatively abundant in the Middle Miocene of Spain. Quaternary applications of SPIRAL have focused on identifying the environmental drivers of C4 grass abundance in tropical East Africa and temperate southeastern Australia over the last 25,000 years. Beyond pollen, SWiM-IRMS is being used to measure the carbon isotopes in individual dissolved chironomid head capsules from Alaskan lakes to quantify the effects of environmental change on lake methane production. The SWiM-IRMS can potentially capture differences in δ13C within and among species that were previously masked by the large sample sizes needed for traditional methods. This also can be used in different microfossils to assess further paleoecological questions integrating several proxies captured from the same organism, such as taxonomy and geochemistry. The potential of SWiM-IRMS in paleoecological studies has likely yet to be fully explored.