RELATING NUTRIENT UPTAKE AND METABOLISM IN AN URBAN CEMENT LINED STREAM
I will accomplish this by measuring the nutrient uptake of ammonia and phosphate as well as metabolism in a cement-lined stream in Bloomington, Illinois during June-August 2019. Metabolism will be measured by deploying dissolved oxygen and photosynthetic light loggers at the stream site. Collected data will be compared to understand if the dominating metabolism of the stream is from algae or microbes in the form of gross primary production (GPP) and community respiration (CR) respectively. The uptake rates will be measured by conducting in-situ uptake analyses and collecting water and biota samples in vesicles appropriate for analyte. Water samples will be tested in a laboratory for ammonia and phosphorus concentrations with an Ion Chromatograph and spectrophotometer, and biota samples will be analyzed for algal and microbial type and density by direct observation and through obtaining the ash free dry mass of samples. The uptake velocities are expected to be high (based on previously collected data), and most of the stream metabolism will be GPP due to high exposure to sunlight. In addition, the dominating biota type is expected to be algae rather than microbes due to GPP being the dominant metabolism.