MICROBIAL COMMUNITY IN HYPERALKALINE STEEL SLAG-FILL EMULATES SERPENTINIZING SPRINGS
In this study, 16S rRNA gene sequences and metagenomic sequence libraries were collected to assess the taxonomic composition and functional potential of microbes present in these slag-polluted waterways. Relative 16S rRNA gene abundances in Calumet sediment and water samples describe community compositions not significantly divergent from those in nearby circumneutral conditions. Major differences in composition are mainly driven by Proteobacteria, primarily one sequence cluster closely related to Hydrogenophaga, which comprises up to 85% of 16S rRNA gene abundance in hyperalkaline surface sediments.
Sequence identity indicates the highly abundant species belongs to the recently established genus Serpentinomonas, a bacterial lineage associated with natural freshwater hyperalkaline serpentinizing springs. Genomic features of Serpentinomonas bacteria indicate they are uniquely adapted to utilizing products of serpentinization, specifically carbonates and hydrogen gas.