DID INCREASING SEASONALITY AND FIRE FREQUENCY CAUSE THE C4 GRASSLAND TRANSITION IN SOUTH AMERICA (SA)? INVESTIGATIONS FROM TWO PALEOSOL SITES IN NW ARGENTINA USING δ13C ISOTOPES, MOLECULAR BIOMARKERS, PHYTOLITHS, AND X-RAY FLUORESCENCE (XRF)

Recent advances in molecular biomarkers along with traditional bulk δ13C isotopic analysis, phytolith assemblages, and XRF analysis allow for the detailed reconstruction of continental paleoecological and paleoclimatic conditions. We use these methods to reconstruct conditions during the expansion of C4 grasses, considered one of the most important biological events during the Cenozoic. We hypothesize that in SA, the spread of C4 grasses was driven by the intensification of the SA Summer Monsoon and that this increase in C4 vegetation caused an intensification of fire regimes, due to both seasonality-fire and fire-C4 expansion feedbacks, that has long been suspected but poorly documented and never studied in this context in SA. This study presents results from well-dated paleosols from two sites in N.W. Argentina: La Viña (LV) and Palo Pintado (PP) in the Salta Province. The LV site includes the Jesus Maria Formations, Guanaco and Piquete (~15 - 4 Ma), while the PP site includes the Palo Pintado Formation (~7.5 - 6 Ma). Previous studies have interpreted these sites as foreland basin sediments deposited in a meandering stream to lacustrine environment. Shifts in the δ13C obtained from bulk organic matter and phytolith assemblages in these paleosols are used to identify vegetation transitions from C3-dominated grasslands and forests, to C4-dominated grasslands. Leaf wax biomarkers include higher order n-alkanes (C20 to C36), and are used to independently determine vegetation composition and changes to aridity and seasonality. XRF analysis is carried out to determine the chemical index of alteration in paleosols, which is a comparable proxy for precipitation seasonality. Polycyclic aromatic hydrocarbons (PAH) are used to determine fire regimes, in order to verify whether C4 expansion promoted fires. δ13C analysis of LV reveals a steady change from a C3-dominated ecosystem at 15 Ma to a C3-C4 intermediary ecosystem by 7.5 Ma. The PP site does not show any directional change in vegetation type during the investigated period between 7.2 Ma to 6 Ma, though δ13C values indicate a mixed C3-C4 ecosystem throughout this period. However, results from the PP site show that increasing C4 cover, determined from bulk organic matter δ13C, is positively correlated with an increased fire frequency, determined from increasing PAH concentrations. Ultimately, these data contribute to constraining the causes of C4 expansion in NW Argentina, including the most important climatic controls and feedbacks from fire regimes, in continental SA.