EXAMINING STATISTICAL RELATIONSHIPS BETWEEN E. COLI AND ANTHROPOGENIC MARKERS IN CHOCCOLOCCO CREEK, AL
We collected water samples once a month from April to September 2021 at nine sample sites throughout the creek. E. coli was measured in triplicate using Coliscan Easygel kits. Turbidity, pH, DO, temperature, and P and N were recorded for each site. The source-specific marker concentrations were determined by solid-phase extraction followed by gas-chromatography mass-spectrometry. Collection of this high dimensional data should allow us to better understand the occurrence of E. coli and source-specific markers in the creek.
Spearman correlations, multiple linear regressions and principal component analysis will be used to identify significant relationships and clusters within the data. Using these analyses, we will will examine the overall watershed along with each individual site. Spatial data including the location of septic tanks, sewer lines, and wastewater outfall will be integrated into our assessment of contamination source. We expect distinct signatures for each of the anthropogenic sources within the watershed which will advance our understanding of fecal contaminant tracing.