Start |Grid View | Author Index | View Uploaded Presentations | Meeting Information

GSA Connects 2023 Meeting in Pittsburgh, Pennsylvania

Paper No. 134-12
Presentation Time: 4:45 PM

THE INHIBITION EFFECT OF ZN ON FUNGAL MN OXIDATION


REN, Wei, Geology, Oklahoma State University, Henry Bellmon Research Center, Stillwater, OK 74078; Forestry, Northwestern A&F University, Taicheng road 3, Yangling, 712100, China, XU, Tingying, Geology, Oklahoma State University, Henry Bellmon Research Center, Stillwater, OK 74078 and TANG, Ming, College of Forestry and Landscape Architecture, South China Agricultural University, State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China

Manganese (Mn) oxides are ubiquitous minerals that commonly control the uptake and release of metals and nutrients and have great potential for metal contaminant remediation. In nature, the oxidation of Mn(II)aq to form the sparingly soluble, nanocrystalline Mn(III/IV) oxides is largely driven by microbial activities through enzymatic and metabolite-mediated pathways. In metal-polluted sites, co-contamination with other metals besides Mn is common. The presence of these additional metals can easily impact the microbial enzymatic and metabolite-mediated pathways, leading to reduced efficiency in removing the metal contaminants by biogenic Mn oxides.

Zinc (Zn) is a widespread co-contaminant commonly found in natural metal-impacted sites. It is an essential element for living organisms but can harm animals and humans at high concentrations. Our previous results have shown that Zn inhibited the formation of Mn oxides without affecting the growth of fungus (a new filament Ascomycota fungus Curvularia lunata TC1). This fungus was isolated from the wastewater at Tar Creek superfund site located in northeast Oklahoma. We have investigated the rate of Mn oxide production by measuring the residual Mn(II) in solution using ICP-OES. Our findings indicated that our fungus was capable of oxidizing 200 µM Mn(II) within 14 days and the presence of a high concentration of Zn (80 µM) inhibited the formation of Mn oxides. This study aims to elucidate the detailed pathway of Zn inhibition of Mn oxidation mediated by fungus TC1. Since Zn participates in lots of biological activities, we assume that Zn may inhibit the activities of specific Mn oxidation-related enzymes in our fungus.

To confirm our hypothesis, we cultivated our fungus in AY media with various amounts of Mn/Zn and collected hyphae at 5 days to identify Mn oxidation-related enzymes. We are conducting transcriptomics analysis using Illumina NovaSeq to find the differentially expressed genes and conducting proteomics analysis using LC/MS/MS to identify Mn oxidation-related enzymes. Results from this study will reveal the pathway of fungus TC1 easily affected by Zn and may provide new insight into the process of biogenic Mn oxidation.

Session No. 134

T131. New Advances in Geobiology
Monday, 16 October 2023: 1:30 PM-5:30 PM
325 (David L Lawrence Convention Center)
Geological Society of America Abstracts with Programs. Vol. 55, No. 6
doi: 10.1130/abs/2023AM-391423
© Copyright 2023 The Geological Society of America (GSA), all rights reserved. Permission is hereby granted to the author(s) of this abstract to reproduce and distribute it freely, for noncommercial purposes. Permission is hereby granted to any individual scientist to download a single copy of this electronic file and reproduce up to 20 paper copies for noncommercial purposes advancing science and education, including classroom use, providing all reproductions include the complete content shown here, including the author information. All other forms of reproduction and/or transmittal are prohibited without written permission from GSA Copyright Permissions.
Back to: T131. New Advances in Geobiology
<< Previous Abstract | Next Abstract >>